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American Society of Microbiology ABMM Sample Questions:

1. The gut microbiome has been implicated in the pathogenesis of autoimmune diseases. Dysbiosis, a disruption in the composition and function of the gut microbiota, can lead to increased intestinal permeability and the translocation of microbial products that trigger inflammatory responses. Which of the following bacterial components is a potent activator of the innate immune system and has been linked to the development of autoimmunity?

A) Flagellin
B) Peptidoglycan
C) Lipopolysaccharide (LPS)
D) Lipoteichoic acid (LTA)

2. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:

A) Candida
B) Mucor
C) Rhizopus
D) Aspergillus

3. A microbiology laboratory receives a CSF sample from a patient with suspected fungal meningitis. India ink staining reveals encapsulated yeast cells. Culture on Sabouraud dextrose agar grows creamy white colonies.
The MOST likely organism is:

A) Aspergillus flavus
B) Cryptococcus neoformans
C) Mucor species
D) Candida albicans

4. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Increased expression of efflux pumps.
B) Production of beta-lactamase enzymes.
C) Acquisition of genes encoding aminoglycoside-modifying enzymes.
D) Alteration of penicillin-binding proteins (PBPs).

5. An acid-fast bacillus isolate recovered from a respiratory specimen after 3 weeks of incubation on Löwenstein- Jensen medium is initially non-pigmented when grown in the dark. After exposure to light for several hours, the colonies develop a distinct yellow-orange pigment. This organism is classified as a:

A) Nonchromogen
B) Rapid grower
C) Scotochromogen
D) Photochromogen

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